How long before seperate puppy from mother dog

8 weeks is the appropriate time to take the puppy away from the mother. If the puppy gets taken too early, they will most likely have some social issues. During that time they learn a lot of things from their mother. "Ethics" pretty much. If a puppy leaves too early they will most likely whine in search of their mommy, and not want to engage with the new family as much. It is best to wait until they are 8 weeks old as they get their shots at 6 weeks. If it is not possible to keep the mother with the puppies till they are 8 weeks, then there may be some issues. I would definitely take them to the vet to get them examined by a professional.